The Human Type IV Collagen (COL4) CLIA Kit is specifically designed for the quantitative determination of Type IV Collagen in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.Type IV Collagen is a key component of the basement membrane, essential for tissue structure and function. Abnormal levels of Type IV Collagen have been linked to various diseases including kidney diseases, certain cancers, and autoimmune disorders.
Therefore, the detection and measurement of Type IV Collagen levels can provide valuable insights into the progression and treatment of these conditions.With its advanced technology and user-friendly format, the Human Type IV Collagen CLIA Kit from Assay Genie is the perfect tool for researchers seeking to investigate the role of Type IV Collagen in health and disease. Order yours today and take your research to the next level.
Product Name:
Human COL4 (Collagen Type IV) CLIA Kit
SKU:
HUES00152
Target:
Human COL4 (Collagen Type IV)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL4. You can calculate the concentration of Human COL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
99-112
101-116
Average (%)
110
106
108
1:4
Range (%)
91-108
102-113
99-111
Average (%)
99
107
105
1:8
Range (%)
88-100
90-104
92-107
Average (%)
95
97
99
1:16
Range (%)
93-105
101-118
84-97
Average (%)
100
108
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
100-112
105
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.44
4.08
0.42
1.46
4.38
Standard deviation
0.04
0.16
0.34
0.05
0.13
0.32
C V (%)
8.7
11.11
8.33
11.9
8.9
7.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL4 in samples. No significant cross-reactivity or interference between Human COL4 and analogues was observed.
