Human COL3 alpha1 (Collagen Type III Alpha 1) ELISA Kit
The Human Col3A1 (Collagen Type III Alpha 1) ELISA Kit is specifically designed for the precise measurement of collagen type III alpha 1 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research purposes.Collagen type III alpha 1 is a key component of the extracellular matrix, playing a vital role in tissue structure, repair, and remodeling.
Dysregulation of collagen type III alpha 1 has been implicated in various diseases such as fibrosis, connective tissue disorders, and cardiovascular conditions, highlighting its importance as a biomarker for disease progression and therapeutic development.By utilizing the Human Col3A1 ELISA Kit, researchers can gain valuable insights into the regulation of collagen type III alpha 1 and its implications in disease pathology, paving the way for innovative diagnostic and treatment strategies.
Product Name:
Human COL3 alpha1 (Collagen Type III Alpha 1) ELISA Kit
SKU:
HUES01916
Target:
Human COL3 alpha1 (Collagen Type III Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL3α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL3α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL3α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL3α1. You can calculate the concentration of Human COL3α1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
97-109
88-101
Average (%)
99
103
93
1:4
Range (%)
100-112
82-95
96-108
Average (%)
106
89
101
1:8
Range (%)
101-117
84-98
91-108
Average (%)
107
90
99
1:16
Range (%)
99-115
87-99
98-110
Average (%)
106
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2
6.4
15.4
2.1
6.5
15.4
Standard deviation
0.1
0.3
0.7
0.1
0.3
0.6
C V (%)
5
4.69
4.55
4.76
4.62
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL3α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL3α1 in samples. No significant cross-reactivity or interference between Human COL3α1 and analogues was observed.
