Human COL2 alpha1 (Collagen Type II Alpha 1) ELISA Kit
The Human Collagen Type II Alpha 1 (COL2A1) ELISA Kit is specifically designed for the accurate detection of COL2A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.COL2A1 is a key structural protein found in cartilage and plays a critical role in maintaining the integrity and function of connective tissues. Dysregulation of COL2A1 expression is associated with various musculoskeletal disorders, such as osteoarthritis and rheumatoid arthritis.
Therefore, this ELISA kit serves as a valuable tool for studying these conditions and potentially developing therapeutic interventions.Overall, the Human Collagen Type II Alpha 1 (COL2A1) ELISA Kit provides researchers with a reliable and effective method for quantifying COL2A1 levels, facilitating advancements in the understanding and treatment of connective tissue-related diseases.
Product Name:
Human COL2 alpha1 (Collagen Type II Alpha 1) ELISA Kit
SKU:
HUES01915
Target:
Human COL2 alpha1 (Collagen Type II Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL2α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL2α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL2α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL2α1. You can calculate the concentration of Human COL2α1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
88-99
87-101
Average (%)
94
94
94
1:4
Range (%)
95-109
82-96
91-104
Average (%)
102
88
97
1:8
Range (%)
98-110
81-91
94-110
Average (%)
104
86
101
1:16
Range (%)
96-114
88-102
98-112
Average (%)
104
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2
4.5
17.4
2.2
4.1
18.6
Standard deviation
0.1
0.3
0.6
0.1
0.2
0.9
C V (%)
5
6.67
3.45
4.55
4.88
4.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL2α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL2α1 in samples. No significant cross-reactivity or interference between Human COL2α1 and analogues was observed.
