Human COL2 alpha1 (Collagen Type II Alpha 1) CLIA Kit
The Human Col2 Alpha1 (Collagen Type II Alpha 1) CLIA Kit is a cutting-edge assay designed for the accurate quantification of Col2 Alpha1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Collagen Type II Alpha 1 is a vital component of cartilage and plays a crucial role in maintaining joint health and function. Abnormal levels of Col2 Alpha1 have been associated with conditions such as osteoarthritis and rheumatoid arthritis, making it a valuable biomarker for studying these diseases and evaluating potential treatment options.
By utilizing the Human Col2 Alpha1 (Collagen Type II Alpha 1) CLIA Kit, researchers can gain valuable insights into the role of Col2 Alpha1 in joint health and disease, ultimately paving the way for advancements in diagnosis, prognosis, and therapeutic interventions.
Product Name:
Human COL2 alpha1 (Collagen Type II Alpha 1) CLIA Kit
SKU:
HUES00487
Target:
Human COL2 alpha1 (Collagen Type II Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL2α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL2α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL2α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL2α1. You can calculate the concentration of Human COL2α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
102-116
88-100
Average (%)
104
109
93
1:4
Range (%)
99-116
102-115
95-111
Average (%)
106
108
103
1:8
Range (%)
87-100
88-101
86-101
Average (%)
92
94
92
1:16
Range (%)
89-101
102-116
88-101
Average (%)
94
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
99-113
107
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
404.0
713.97
3267.82
405.12
777.88
3344.09
Standard deviation
50.3
70.18
341.16
45.78
86.73
358.49
C V (%)
12.45
9.83
10.44
11.3
11.15
10.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL2α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL2α1 in samples. No significant cross-reactivity or interference between Human COL2α1 and analogues was observed.
