The Human COL13 (Collagen Type 13) ELISA Kit is a reliable and accurate tool for the quantification of collagen type 13 levels in human samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for precise and consistent results.Collagen type 13 is a key component of the extracellular matrix, playing a critical role in tissue organization and maintenance. Abnormal levels of collagen type 13 have been associated with various pathological conditions, including connective tissue disorders and fibrosis.
Therefore, this ELISA kit is essential for studying the role of collagen type 13 in health and disease, facilitating research in areas such as wound healing, tissue regeneration, and fibrotic diseases.Overall, the Human COL13 ELISA Kit is a valuable tool for researchers in the fields of cell biology, biochemistry, and molecular biology, providing a reliable and efficient method for measuring collagen type 13 levels in human samples.
Product Name:
Human COL13 (Collagen Type 13) ELISA Kit
SKU:
HUES01999
Target:
Human COL13 (Collagen Type 13)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL13. You can calculate the concentration of Human COL13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
87-99
90-101
Average (%)
97
93
96
1:4
Range (%)
91-107
81-93
87-101
Average (%)
98
87
94
1:8
Range (%)
94-105
83-97
82-97
Average (%)
100
89
89
1:16
Range (%)
89-102
86-100
84-94
Average (%)
95
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.51
4.39
0.47
1.64
4.36
Standard deviation
0.03
0.08
0.16
0.03
0.07
0.21
C V (%)
5.88
5.3
3.64
6.38
4.27
4.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL13 in samples. No significant cross-reactivity or interference between Human COL13 and analogues was observed.
