Human COL1 alpha2 (Collagen Type I Alpha 2) ELISA Kit
The Human COL1A2 (Collagen Type I Alpha 2) ELISA Kit is specifically designed for the precise measurement of collagen type I alpha 2 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and dependable results for a variety of research applications.Collagen type I alpha 2 is a vital component of the extracellular matrix, contributing to the structural integrity of tissues such as skin, tendons, and bone.
Dysregulation of collagen synthesis and degradation is associated with numerous pathological conditions, including fibrosis, arthritis, and skin disorders. Therefore, this ELISA kit serves as a valuable tool for investigating collagen-related diseases and developing potential therapeutic interventions.
Product Name:
Human COL1 alpha2 (Collagen Type I Alpha 2) ELISA Kit
SKU:
HUES01914
Target:
Human COL1 alpha2 (Collagen Type I Alpha 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL1α2. You can calculate the concentration of Human COL1α2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
99-112
93-108
Average (%)
105
105
100
1:4
Range (%)
86-100
83-96
87-101
Average (%)
92
88
93
1:8
Range (%)
90-101
85-100
84-96
Average (%)
96
92
91
1:16
Range (%)
89-104
83-95
84-97
Average (%)
96
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.59
4.86
0.56
1.45
5.11
Standard deviation
0.03
0.07
0.15
0.03
0.07
0.26
C V (%)
5.77
4.4
3.09
5.36
4.83
5.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL1α2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL1α2 in samples. No significant cross-reactivity or interference between Human COL1α2 and analogues was observed.
