Human COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
The Human Col1 Alpha2 (Collagen Type I Alpha 2) CLIA Kit is specially designed for the precise measurement of Col1 Alpha2 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Col1 Alpha2 is an important component of collagen type I, a structural protein that provides strength and support to tissues in the body, including skin, bone, and cartilage. Abnormal levels of Col1 Alpha2 have been associated with conditions such as osteogenesis imperfecta, Ehlers-Danlos syndrome, and fibrosis, making it a valuable biomarker for studying these disorders and exploring potential treatments.
With the Human Col1 Alpha2 CLIA Kit, researchers can gain valuable insights into the role of collagen type I in health and disease, paving the way for new discoveries and therapeutic interventions. Trust in this innovative kit for accurate and reliable Col1 Alpha2 measurements in your research projects.
Product Name:
Human COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
SKU:
HUES00486
Target:
Human COL1 alpha2 (Collagen Type I Alpha 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL1α2. You can calculate the concentration of Human COL1α2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
101-114
100-115
Average (%)
93
108
106
1:4
Range (%)
88-101
87-98
100-114
Average (%)
95
92
106
1:8
Range (%)
94-107
103-115
87-104
Average (%)
101
109
95
1:16
Range (%)
93-109
100-113
92-104
Average (%)
100
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.56
189.05
753.17
109.77
198.0
744.85
Standard deviation
12.43
16.07
48.73
12.43
14.59
64.36
C V (%)
12.0
8.5
6.47
11.32
7.37
8.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL1α2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL1α2 in samples. No significant cross-reactivity or interference between Human COL1α2 and analogues was observed.
