Human CNTF Recombinant Protein (RPPB0129)
- SKU:
- RPPB0129
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- CNTF
- Synonyms:
- Ciliary neurotrophic factor
- CNTF
- HCNTF
- Source:
- Escherichia Coli
- Uniprot:
- P26441
Description
| Product Name: | Human CNTF Recombinant Protein |
| Product Code: | RPPB0129 |
| Size: | 20µg |
| Species: | Human |
| Target: | CNTF |
| Synonyms: | Ciliary neurotrophic factor, CNTF, HCNTF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | CNTF protein solution (1mg/ml) containing 20 mM Tris-HClbuffer (pH 8.5), 1 mM DTT,30% Glycerol and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSHMAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEADGMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Biological Activity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is lessor equal to 1 ug/ml. |
CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
CNTF Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 220 amino acids (1-200 a.a) andhaving a molecular mass of 25kDa. CNTF is fused to a 20 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 11q12.2 Cellular Component: cytoplasm; extracellular region; extracellular space Molecular Function:ciliary neurotrophic factor receptor binding; growth factor activity; interleukin-6 receptor binding; protein binding Biological Process: growth; muscle morphogenesis; negative regulation of neuron apoptosis; negative regulation of photoreceptor cell differentiation; neuron development; positive regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of retinal cell programmed cell death; signal transduction |
| NCBI Summary: | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| UniProt Code: | P26441 |
| NCBI GenInfo Identifier: | 116585 |
| NCBI Gene ID: | 1270 |
| NCBI Accession: | P26441.1 |
| UniProt Secondary Accession: | P26441,B2RAB2, |
| UniProt Related Accession: | P26441 |
| Molecular Weight: | |
| NCBI Full Name: | Ciliary neurotrophic factor |
| NCBI Synonym Full Names: | ciliary neurotrophic factor |
| NCBI Official Symbol: | CNTF |
| NCBI Official Synonym Symbols: | HCNTF |
| NCBI Protein Information: | ciliary neurotrophic factor |
| UniProt Protein Name: | Ciliary neurotrophic factor |
| Protein Family: | Ciliary neurotrophic factor |
| UniProt Gene Name: | CNTF |
| UniProt Entry Name: | CNTF_HUMAN |
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