Human CNR1 (Cannabinoid Receptor 1, Brain) ELISA Kit
The Human CNR1 (Cannabinoid Receptor 1) Brain ELISA Kit is specifically designed for the accurate detection of CNR1 levels in human brain tissue samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Cannabinoid Receptor 1 plays a vital role in the endocannabinoid system, regulating various physiological processes such as pain sensation, mood, appetite, and memory. Dysfunction of the CNR1 receptor has been linked to a range of conditions including neurodegenerative disorders, psychiatric disorders, and chronic pain syndromes, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its reliable performance and ease of use, the Human CNR1 Brain ELISA Kit is an indispensable tool for researchers studying the role of the endocannabinoid system in health and disease.
Product Name:
Human CNR1 (Cannabinoid Receptor 1, Brain) ELISA Kit
SKU:
HUES01797
Target:
Human CNR1 (Cannabinoid Receptor 1, Brain)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CNR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CNR1. You can calculate the concentration of Human CNR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
87-98
83-98
Average (%)
96
93
90
1:4
Range (%)
89-100
80-94
88-99
Average (%)
94
86
94
1:8
Range (%)
89-102
85-97
85-101
Average (%)
96
92
92
1:16
Range (%)
88-103
83-97
85-96
Average (%)
95
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.2
3.59
0.55
1.31
3.89
Standard deviation
0.03
0.05
0.14
0.03
0.06
0.16
C V (%)
5.88
4.17
3.9
5.45
4.58
4.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CNR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CNR1 in samples. No significant cross-reactivity or interference between Human CNR1 and analogues was observed.
