CNDP1; also known as carnosine dipeptidase 1; glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1); is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region; which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy; for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans; CNDP1 is secreted from the liver into the serum. In other mammals; including rodents; CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β-alanyl-L-histidine); and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans; but not African-Americans. It was identified and confirmed as a risk factor; were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition; CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.
Measured by its ability to cleave carnosine (?AlaLHis) in a twostep assay.The specific activity is > 250 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human CNDP1 (NP_116038.4) (Ser27-His507) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
55.3 kDa
AP Mol Mass:
60-65 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
