Human Cluster Of Differentiation 226 (CD226) ELISA Kit
The Human Cluster of Differentiation 226 (CD226) ELISA Kit is specifically designed for the precise measurement of CD226 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for various research applications.CD226, also known as DNAM-1, is a key protein involved in immune responses and cell adhesion processes. It plays a crucial role in regulating immune cell function and promoting cell-to-cell interactions.
Dysregulation of CD226 has been implicated in autoimmune diseases, inflammatory disorders, and cancer progression, highlighting its importance as a potential biomarker for disease monitoring and therapeutic development.Overall, the Human CD226 ELISA Kit provides researchers with a reliable and efficient method to study the role of CD226 in human health and disease, opening up new avenues for biomedical research and therapeutic interventions.
Product Name:
Human Cluster Of Differentiation 226 (CD226) ELISA Kit
Product Code:
HUDL00484
Size:
96 Assays
Target:
CD226
Synonyms:
DNAM1, PTA1, TLiSA1, DNAX accessory molecule 1
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
