The Human CLDN3 (Claudin-3) ELISA Kit offered by Assay Genie is a premier tool for the precise measurement of CLDN3 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for a variety of research endeavors.CLDN3, a member of the claudin family, plays a crucial role in maintaining the integrity of tight junctions between cells and regulating the permeability of epithelial barriers.
Dysregulation of CLDN3 has been implicated in various diseases, including cancer and inflammatory disorders, highlighting its significance as a potential biomarker for disease detection and progression.By utilizing the Human CLDN3 ELISA Kit, researchers can gain valuable insight into the role of CLDN3 in health and disease, paving the way for innovative treatment strategies and personalized medicine approaches. Trust Assay Genie for high-quality ELISA kits that drive groundbreaking discoveries in biomedical research.
Product Name:
Human CLDN3 (Claudin 3) ELISA Kit
SKU:
HUES01898
Target:
Human CLDN3 (Claudin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLDN3. You can calculate the concentration of Human CLDN3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
95-106
90-105
Average (%)
91
100
96
1:4
Range (%)
88-103
80-93
87-102
Average (%)
95
85
94
1:8
Range (%)
89-105
88-98
86-97
Average (%)
97
93
92
1:16
Range (%)
88-103
83-94
88-101
Average (%)
96
89
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.88
7.66
1.04
2.9
8.21
Standard deviation
0.06
0.16
0.28
0.07
0.12
0.44
C V (%)
6.06
5.56
3.66
6.73
4.14
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLDN3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN3 in samples. No significant cross-reactivity or interference between Human CLDN3 and analogues was observed.
