The Human CLDN3 (Claudin-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CLDN3 levels in human serum, plasma, and cell culture supernatants. CLDN3 is a vital protein involved in cell adhesion and tight junction formation, playing a crucial role in maintaining the integrity of epithelial barriers.This CLIA kit offers reliable and reproducible results, making it a valuable tool for researchers studying conditions such as cancer, inflammatory bowel disease, and hepatitis where CLDN3 dysregulation has been implicated.
By accurately quantifying CLDN3 levels, this kit can help researchers gain insights into disease mechanisms and potentially identify new therapeutic targets.
Product Name:
Human CLDN3 (Claudin 3) CLIA Kit
SKU:
HUES00473
Target:
Human CLDN3 (Claudin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLDN3. You can calculate the concentration of Human CLDN3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
97-110
87-99
Average (%)
98
103
93
1:4
Range (%)
90-102
90-104
99-115
Average (%)
95
97
107
1:8
Range (%)
95-109
89-101
92-105
Average (%)
103
96
99
1:16
Range (%)
86-101
92-107
90-104
Average (%)
93
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-118
108
EDTA plasma (n=5)
90-108
98
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.74
455.48
1365.93
223.75
475.1
1337.52
Standard deviation
18.25
32.84
107.5
22.84
37.49
138.7
C V (%)
8.38
7.21
7.87
10.21
7.89
10.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLDN3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN3 in samples. No significant cross-reactivity or interference between Human CLDN3 and analogues was observed.
