The Human CLDN1 (Claudin-1) ELISA Kit is specifically designed to accurately detect levels of CLDN1 in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it ideal for various research applications.Claudin-1 is a vital protein that plays a role in cell adhesion and the maintenance of tight junctions in epithelial cells.
Dysregulation of CLDN1 has been associated with various diseases, including cancer and inflammatory bowel disease. As such, this ELISA kit serves as a valuable tool in studying the role of CLDN1 in disease progression and in developing potential therapeutic interventions.
Product Name:
Human CLDN1 (Claudin 1) ELISA Kit
SKU:
HUES01890
Target:
Human CLDN1 (Claudin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLDN1. You can calculate the concentration of Human CLDN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
98-115
90-104
Average (%)
99
105
98
1:4
Range (%)
89-104
83-94
86-99
Average (%)
96
89
93
1:8
Range (%)
94-108
84-96
87-101
Average (%)
100
89
92
1:16
Range (%)
92-107
82-95
84-96
Average (%)
98
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.14
4.11
0.53
1.25
3.96
Standard deviation
0.03
0.05
0.13
0.04
0.05
0.2
C V (%)
6
4.39
3.16
7.55
4.0
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLDN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN1 in samples. No significant cross-reactivity or interference between Human CLDN1 and analogues was observed.
