Human CLC (Charcot Leyden Crystal Protein) ELISA Kit
The Human CLC (Charcot-Leyden Crystal) Protein ELISA Kit is specifically designed for the accurate and sensitive detection of CLC protein levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring reliable and reproducible results for a variety of research applications.The CLC protein, also known as galectin-10, is a key player in allergic inflammation and asthma. It is associated with the formation of Charcot-Leyden crystals, which are often found in the sputum of patients with bronchial asthma.
By measuring CLC protein levels, researchers can gain valuable insights into the pathogenesis of allergic diseases and potentially develop new therapeutic strategies.Overall, the Human CLC Protein ELISA Kit provides a valuable tool for researchers studying allergic inflammation, asthma, and related conditions, offering a convenient and reliable way to quantify CLC protein levels in human samples.
Product Name:
Human CLC (Charcot Leyden Crystal Protein) ELISA Kit
SKU:
HUES02539
Target:
Human CLC (Charcot Leyden Crystal Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLC. You can calculate the concentration of Human CLC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
85-98
87-103
Average (%)
94
90
94
1:4
Range (%)
91-104
84-99
83-95
Average (%)
97
91
88
1:8
Range (%)
89-101
80-91
88-100
Average (%)
94
85
93
1:16
Range (%)
91-105
80-92
84-95
Average (%)
97
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.45
4.74
0.5
1.57
4.51
Standard deviation
0.03
0.09
0.15
0.03
0.07
0.22
C V (%)
6.25
6.21
3.16
6.0
4.46
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLC in samples. No significant cross-reactivity or interference between Human CLC and analogues was observed.
