The Human CKM (Creatine Kinase Muscle) ELISA Kit is specifically designed for the precise measurement of CKM levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for various research applications.CKM is an important enzyme found primarily in muscle tissue, playing a key role in energy metabolism and muscle contraction.
Abnormal levels of CKM are associated with various muscle disorders, making it a valuable biomarker for studying muscular diseases and monitoring treatment efficacy.With the Human CKM ELISA Kit, researchers can easily analyze CKM levels in biological samples, gaining valuable insights into muscle health and potentially identifying new therapeutic targets for muscle-related conditions.
Product Name:
Human CKM (Creatine Kinase, Muscle) ELISA Kit
SKU:
HUES02469
Target:
Human CKM (Creatine Kinase, Muscle)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CKM. You can calculate the concentration of Human CKM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
90-103
98-114
Average (%)
98
96
104
1:4
Range (%)
88-104
87-101
88-99
Average (%)
95
92
93
1:8
Range (%)
88-101
83-94
88-98
Average (%)
95
88
93
1:16
Range (%)
93-106
84-96
88-102
Average (%)
98
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
97-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.27
13.82
46.55
5.26
12.97
50.31
Standard deviation
0.36
0.58
1.55
0.27
0.54
2.65
C V (%)
6.83
4.2
3.33
5.13
4.16
5.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CKM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKM in samples. No significant cross-reactivity or interference between Human CKM and analogues was observed.
