Human CKB Recombinant Protein (RPPB2479)
- SKU:
- RPPB2479
- Product type:
- Recombinant Protein
- Size:
- 50ug
- Species:
- Human
- Target:
- CKB
- Synonyms:
- Creatine kinase B-type
- EC 2732
- Creatine kinase B chain
- B-CK
- Source:
- Pichia Pastoris
- Uniprot:
- P12277
Description
| Product Name: | Human CKB Recombinant Protein |
| Product Code: | RPPB2479 |
| Size: | 50µg |
| Species: | Human |
| Target: | CKB |
| Synonyms: | Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI. |
| Source: | Pichia Pastoris |
| Physical Appearance: | Sterile Filtered colourless liquid formulation. |
| Formulation: | CKBB Human contains 10Mm Bis-Tris-HCl pH-6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT. |
| Stability: | CKBB should be stored below -18°C. Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Biological Activity: | The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml. |
Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
CKBB Human Recombinant produced in Pichia Pastoris is a dimeric glycosylated full length polypeptide chain comprised of 2 identical B subunits and having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and having a Mw of 47kDaThe CKBB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. |
| UniProt Protein Details: | Catalytic activity: ATP + creatine = ADP + phosphocreatine. Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Subcellular location: Cytoplasm. Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain. |
| NCBI Summary: | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq] |
| UniProt Code: | P12277 |
| NCBI GenInfo Identifier: | 125294 |
| NCBI Gene ID: | 1152 |
| NCBI Accession: | P12277.1 |
| UniProt Secondary Accession: | P12277,Q2LE07, Q6FG40, Q9UC66, A8K236, B2R5R4, |
| UniProt Related Accession: | P12277 |
| Molecular Weight: | 42,644 Da |
| NCBI Full Name: | Creatine kinase B-type |
| NCBI Synonym Full Names: | creatine kinase, brain |
| NCBI Official Symbol: | CKB |
| NCBI Official Synonym Symbols: | B-CK; CKBB |
| NCBI Protein Information: | creatine kinase B-type; creatine kinase-B; creatine kinase B chain |
| UniProt Protein Name: | Creatine kinase B-type |
| UniProt Synonym Protein Names: | B-CK; Creatine kinase B chain |
| Protein Family: | Creatine kinase |
| UniProt Gene Name: | CKB |
| UniProt Entry Name: | KCRB_HUMAN |
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