Human Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) ELISA Kit

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SKU:
HUDL00568
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P36544
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CHR-NA7,CHRN-A7,N-AChRA7,NAChRA7,N-AChR-A7,Neuronal Acetylcholine Receptor Alpha 7
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) ELISA Kit

The Human Cholinergic Receptor Nicotinic Alpha 7 (CHRNA7) ELISA Kit is designed for the precise measurement of CHRNA7 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.CHRNA7 is a critical receptor involved in cholinergic signaling, playing a key role in cognitive function, memory, and neurological disorders such as schizophrenia and Alzheimer's disease.

By detecting and quantifying CHRNA7 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human CHRNA7 ELISA Kit provides researchers with a powerful tool for studying the role of cholinergic receptors in health and disease, paving the way for advancements in neuroscience and drug discovery.

Product Name:Human Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) ELISA Kit
Product Code:HUDL00568
Size:96 Assays
Target:CHRNa7
Synonyms:CHR-NA7, CHRN-A7, N-AChRA7, NAChRA7, N-AChR-A7, Neuronal Acetylcholine Receptor Alpha 7
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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