Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit

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SKU:
HUDL00565
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02708
Sensitivity:
0.28ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CHR-NA1,N-AChRA1,NAChRA1,ACHRA,ACHRD,CMS2A,FCCMS,SCCMS,Acetylcholine receptor subunit alpha,N-AChR-A1,Neuronal Acetylcholine Receptor Alpha 1,Muscle
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit

The Human Cholinergic Receptor Nicotinic Alpha 1 (CHRNA1) ELISA Kit is specially designed for the accurate measurement of CHRNA1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CHRNA1 is a vital receptor that plays a crucial role in the cholinergic system, influencing neurotransmission and muscle function.

Dysregulation of CHRNA1 has been linked to various neurological disorders, making it a valuable biomarker for investigating these conditions and potentially developing therapeutic interventions.By using the Human CHRNA1 ELISA Kit, researchers can gain valuable insights into the role of this important receptor in health and disease, advancing our understanding of neurological disorders and paving the way for innovative treatments.

Product Name:Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit
Product Code:HUDL00565
Size:96 Assays
Target:CHRNa1
Synonyms:CHR-NA1, N-AChRA1, NAChRA1, ACHRA, ACHRD, CMS2A, FCCMS, SCCMS, Acetylcholine receptor subunit alpha, N-AChR-A1, Neuronal Acetylcholine Receptor Alpha 1,Muscle
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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