Human Cholecystokinin A Receptor (CCKAR) ELISA Kit
The Human Cholecystokinin-A Receptor (CCKAR) ELISA Kit is a reliable tool for the sensitive detection of CCKAR levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, enabling researchers to obtain consistent and trustworthy results for various research applications.The CCKAR is a crucial receptor involved in mediating the effects of cholecystokinin, a peptide hormone important for regulating digestion and appetite. Dysregulation of the CCKAR signaling pathway has been implicated in various gastrointestinal disorders, making it a key target for studying and developing potential treatments for these conditions.
With its high sensitivity and specificity, the Human CCKAR ELISA Kit is a valuable tool for researchers investigating the role of CCKAR in health and disease. Its reliable performance and precise measurements make it an essential asset for advancing our understanding of CCKAR biology and its potential implications in therapeutic interventions.
Product Name:
Human Cholecystokinin A Receptor (CCKAR) ELISA Kit
Product Code:
HUDL00456
Size:
96 Assays
Target:
CCKAR
Synonyms:
CCK-A, CCK1, CCKRA, CCK1R, Cholecystokinin Type 1 Receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
