Human Chloride Intracellular Channel Protein 1 (CLIC1) ELISA Kit
The Human CLIC1 (Chloride Intracellular Channel Protein 1) ELISA Kit is specifically designed for the precise measurement of CLIC1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results that are suitable for a wide range of research purposes.CLIC1 is a vital protein that functions as an ion channel and is implicated in various cellular processes, including cell proliferation, apoptosis, and intracellular chloride transport.
Its dysregulation has been linked to multiple diseases, such as cancer, autoimmune disorders, and neurological conditions, underscoring its importance as a biomarker for studying these diseases and developing potential therapeutic interventions.Order the Human CLIC1 ELISA Kit today to unlock valuable insights into the role of CLIC1 in disease pathogenesis and progression.
Product Name:
Human Chloride Intracellular Channel Protein 1 (CLIC1) ELISA Kit
Product Code:
HUDL00596
Size:
96 Assays
Target:
CLIC1
Synonyms:
G6, NCC27, p64CLCP, hRNCC, Chloride channel ABP, Nuclear chloride ion channel 27, Regulatory nuclear chloride ion channel protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
