The Human CHGA (Chromogranin A) ELISA Kit is specially designed for the accurate measurement of Chromogranin A levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it an indispensable tool for a wide range of research applications.Chromogranin A is a vital protein that plays a key role in various physiological processes, including neurotransmitter release and hormone secretion.
It is implicated in a number of diseases such as neuroendocrine tumors, pheochromocytoma, and heart failure, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.This Human CHGA ELISA Kit provides researchers with a powerful tool to accurately quantify Chromogranin A levels, enabling a deeper understanding of its role in health and disease. Order now and unlock the potential of Chromogranin A in your research endeavors.
Product Name:
Human CHGA (Chromogranin A) ELISA Kit
SKU:
HUES01884
Target:
Human CHGA (Chromogranin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHGA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHGA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHGA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CHGA. You can calculate the concentration of Human CHGA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
84-99
93-104
Average (%)
102
91
99
1:4
Range (%)
88-101
85-100
85-99
Average (%)
93
91
91
1:8
Range (%)
91-103
86-101
87-100
Average (%)
96
92
92
1:16
Range (%)
91-103
86-98
87-100
Average (%)
98
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.03
517.46
1372.57
165.11
549.96
1383.06
Standard deviation
12.67
27.58
71.24
11.19
29.15
51.45
C V (%)
6.92
5.33
5.19
6.78
5.3
3.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CHGA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHGA in samples. No significant cross-reactivity or interference between Human CHGA and analogues was observed.
