Human Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) ELISA Kit
The Human Chemokine C-X3-C Motif Receptor 1 (CX3CR1) ELISA Kit is a powerful tool for detecting levels of CX3CR1 in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and precise results for a variety of research applications. CX3CR1 is a key receptor involved in regulating immune cell trafficking and inflammation, making it a valuable target for studying conditions such as autoimmune diseases, inflammatory disorders, and neuroinflammatory diseases.
By accurately measuring CX3CR1 levels, researchers can gain insights into disease pathogenesis and potentially identify new therapeutic strategies. Whether you are investigating the role of CX3CR1 in disease progression or developing targeted therapies, the Human CX3CR1 ELISA Kit offers the reliability and performance needed for successful research outcomes. Order your kit today and unlock the potential of CX3CR1 in your scientific studies.
Product Name:
Human Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
