Human Chemokine C-X-C-Motif Receptor 6 (CXCR6) ELISA Kit

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SKU:
HUDL00751
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00574
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD186,BONZO,STRL33,TYMSTR,G-protein coupled receptor STRL33,G-protein coupled receptor bonzo
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Human Chemokine C-X-C-Motif Receptor 6 (CXCR6) ELISA Kit

The Human C-X-C Motif Receptor 6 (CXCR6) ELISA Kit is a powerful tool for the precise measurement of CXCR6 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, catering to a wide range of research needs.CXCR6 is a key receptor involved in immune response and cell migration, playing a vital role in various physiological and pathological processes.

Its dysregulation has been linked to conditions like inflammation, autoimmune diseases, and cancer, highlighting its importance as a potential therapeutic target and diagnostic marker.Investigate the intricate mechanisms of immune modulation and disease progression with confidence using the Human CXCR6 ELISA Kit, providing valuable insights for biomedical research and clinical applications.

Product Name:Human Chemokine C-X-C-Motif Receptor 6 (CXCR6) ELISA Kit
Product Code:HUDL00751
Size:96 Assays
Target:CXCR6
Synonyms:CD186, BONZO, STRL33, TYMSTR, G-protein coupled receptor STRL33, G-protein coupled receptor bonzo
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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