Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit

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SKU:
HUDL00749
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P61073
Sensitivity:
0.060ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD184,Fusin,NPYR,HSY3RR,LAP3,LCR1,LESTR,NPY3R,NPYRL,WHIM,Leukocyte-derived seven transmembrane domain receptor,Lipopolysaccharide-associated protein 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit

The Human Chemokine C-X-C Motif Receptor 4 (CXCR4) ELISA Kit is specifically designed for the precise detection of CXCR4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research purposes.CXCR4 is a pivotal chemokine receptor that plays a critical role in various physiological and pathological processes, including immune response, inflammation, and cancer metastasis.

By measuring CXCR4 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and identify potential therapeutic targets.With its reliable performance and versatility, the Human CXCR4 ELISA Kit is an indispensable tool for studying the role of CXCR4 in health and disease, and for advancing novel research and therapeutic strategies.

Product Name:Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit
Product Code:HUDL00749
Size:96 Assays
Target:CXCR4
Synonyms:CD184, Fusin, NPYR, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYRL, WHIM, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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