Human Chemokine C-C-Motif Receptor 6 (CCR6) ELISA Kit
The Human CCR6 ELISA Kit is a cutting-edge tool specifically designed for the precise measurement of CCR6 levels in human serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, guaranteeing consistent and accurate results for a diverse array of research endeavors.CCR6 is a vital chemokine receptor known to play a pivotal role in immune responses and inflammation. By facilitating the migration and activation of immune cells, CCR6 contributes significantly to the body's defense mechanisms against pathogens and diseases. Understanding CCR6 levels can offer invaluable insights into various inflammatory conditions, autoimmune disorders, and cancer, making it a crucial biomarker for studying and potentially treating these conditions.
With its advanced technology and reliability, the Human CCR6 ELISA Kit is the perfect solution for researchers seeking to delve deeper into the intricate workings of the immune system and inflammatory processes. Trust in this kit to provide you with accurate and reproducible data, paving the way for groundbreaking discoveries in the field of immunology and beyond.
Product Name:
Human Chemokine C-C-Motif Receptor 6 (CCR6) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
