Human CFL2 Recombinant Protein (RPPB3159)
- SKU:
- RPPB3159
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- CFL2
- Synonyms:
- Cofilin-2
- Cofilin- muscle isoform
- CFL2
- NEM7
- Source:
- Escherichia Coli
- Uniprot:
- Q9Y281
Description
| Product Name: | Human CFL2 Recombinant Protein |
| Product Code: | RPPB3159 |
| Size: | 20µg |
| Species: | Human |
| Target: | CFL2 |
| Synonyms: | Cofilin-2, Cofilin- muscle isoform, CFL2, NEM7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CFL2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL |
CFL2 protein is a member of the actin-binding proteins ADF family which contains 1 ADF-H domain. Cofilin is a broadly distributed intracellular actin-modulating protein which binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in the CFL2 gene are the cause of nemaline myopathy type 7 (NEM7).
CFL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-166 a.a.) and having a molecular mass of 20.9kDa.CFL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 14q12 Cellular Component: extracellular space; I band Molecular Function:protein binding Biological Process: positive regulation of actin filament depolymerization Disease: Nemaline Myopathy 7 |
| NCBI Summary: | This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009] |
| UniProt Code: | Q9Y281 |
| NCBI GenInfo Identifier: | 6831517 |
| NCBI Gene ID: | 1073 |
| NCBI Accession: | Q9Y281.1 |
| UniProt Secondary Accession: | Q9Y281,G3V5P4, |
| UniProt Related Accession: | Q9Y281 |
| Molecular Weight: | – Da |
| NCBI Full Name: | Cofilin-2 |
| NCBI Synonym Full Names: | cofilin 2 |
| NCBI Official Symbol: | CFL2 |
| NCBI Official Synonym Symbols: | NEM7 |
| NCBI Protein Information: | cofilin-2 |
| UniProt Protein Name: | Cofilin-2 |
| UniProt Synonym Protein Names: | Cofilin, muscle isoform |
| Protein Family: | Cofilin |
| UniProt Gene Name: | CFL2 |
| UniProt Entry Name: | COF2_HUMAN |
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