Human CFL2 Recombinant Protein (RPPB3159)

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SKU:
RPPB3159
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
CFL2
Synonyms:
Cofilin-2
Synonyms:
Cofilin- muscle isoform
Synonyms:
CFL2
Synonyms:
NEM7
Source:
Escherichia Coli
Uniprot:
Q9Y281
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Description

Product Name:Human CFL2 Recombinant Protein
Product Code:RPPB3159
Size:20µg
Species:Human
Target:CFL2
Synonyms:Cofilin-2, Cofilin- muscle isoform, CFL2, NEM7.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:CFL2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 50mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL

CFL2 protein is a member of the actin-binding proteins ADF family which contains 1 ADF-H domain. Cofilin is a broadly distributed intracellular actin-modulating protein which binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in the CFL2 gene are the cause of nemaline myopathy type 7 (NEM7).

CFL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-166 a.a.) and having a molecular mass of 20.9kDa.CFL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: extracellular space; I band

Molecular Function:protein binding

Biological Process: positive regulation of actin filament depolymerization

Disease: Nemaline Myopathy 7

NCBI Summary:This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
UniProt Code:Q9Y281
NCBI GenInfo Identifier:6831517
NCBI Gene ID:1073
NCBI Accession:Q9Y281.1
UniProt Secondary Accession:Q9Y281,G3V5P4,
UniProt Related Accession:Q9Y281
Molecular Weight:– Da
NCBI Full Name:Cofilin-2
NCBI Synonym Full Names:cofilin 2
NCBI Official Symbol:CFL2  
NCBI Official Synonym Symbols:NEM7  
NCBI Protein Information:cofilin-2
UniProt Protein Name:Cofilin-2
UniProt Synonym Protein Names:Cofilin, muscle isoform
Protein Family:Cofilin
UniProt Gene Name:CFL2  
UniProt Entry Name:COF2_HUMAN
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