The Human CFD (Complement Factor D) CLIA Kit is a reliable tool for the precise measurement of Complement Factor D levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Complement Factor D is a vital component of the complement system, a key part of the immune response that helps to enhance inflammation and eliminate pathogens. Abnormal levels of Complement Factor D have been associated with various inflammatory diseases, autoimmune disorders, and infections, making it a valuable biomarker for understanding these conditions and developing potential treatments.
This Human CFD CLIA Kit provides researchers with a comprehensive solution for studying the role of Complement Factor D in health and disease. Its high performance and user-friendly design make it an essential tool for advancing scientific knowledge and discovering new therapeutic strategies.
Product Name:
Human CFD (Complement Factor D) CLIA Kit
SKU:
HUES00040
Target:
Human CFD (Complement Factor D)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CFD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CFD. You can calculate the concentration of Human CFD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
96-111
95-112
Average (%)
95
101
103
1:4
Range (%)
86-98
101-117
92-109
Average (%)
91
109
99
1:8
Range (%)
99-112
86-97
100-113
Average (%)
105
92
107
1:16
Range (%)
85-95
89-103
100-115
Average (%)
90
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.24
687.31
2486.95
242.57
721.32
2632.72
Standard deviation
22.39
49.56
250.68
31.39
85.19
235.89
C V (%)
9.6
7.21
10.08
12.94
11.81
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CFD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed.
