Human CENPB Recombinant Protein (RPPB3145)
- SKU:
- RPPB3145
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- CENPB
- Synonyms:
- Major centromere autoantigen B
- Centromere protein B
- CENP-B
- CENPB
- Source:
- Sf9 Insect cells
- Uniprot:
- P07199
Description
| Product Name: | Human CENPB Recombinant Protein |
| Product Code: | RPPB3145 |
| Size: | 20µg |
| Species: | Human |
| Target: | CENPB |
| Synonyms: | Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered liquid formulation. |
| Formulation: | CENPB is supplied at a concentration of in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 20p13 Cellular Component: chromosome; nucleus; chromosome, pericentric region Molecular Function:centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding Biological Process: regulation of transcription, DNA-dependent |
| UniProt Code: | P07199 |
| NCBI GenInfo Identifier: | 29863 |
| NCBI Gene ID: | |
| NCBI Accession: | CAA28918.1 |
| Molecular Weight: | 80kD |
| NCBI Full Name: | CENP-B |
| UniProt Protein Name: | Major centromere autoantigen B |
| UniProt Synonym Protein Names: | Centromere protein B |
| Protein Family: | CENP-B homolog protein |
| UniProt Gene Name: | CENPB |
| UniProt Entry Name: | CENPB_HUMAN |
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