Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit
The Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit is a powerful tool for measuring the levels of CDC42 in human samples. CDC42 is a key regulator of cell division and plays a vital role in various cellular processes such as cell migration, proliferation, and differentiation.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers studying cell cycle regulation, cancer development, and related signaling pathways.
By accurately quantifying CDC42 levels in serum, plasma, or cell culture supernatants, researchers can gain valuable insights into the function of this important protein and its role in disease progression.With its easy-to-use format and robust performance, the Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit is an essential tool for advancing research in cell biology, cancer biology, and drug development. Order yours today and take your research to the next level.
Product Name:
Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit
Product Code:
HUDL00502
Size:
96 Assays
Target:
CDC42
Synonyms:
CDC42Hs, G25K, G25K GTP-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
