The Human CECR1 (Cat Eye Syndrome Chromosome Region, Candidate 1) ELISA Kit is specifically designed for the accurate measurement of CECR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CECR1 is a key protein implicated in various biological processes, including chromatin remodeling and gene expression regulation.
Dysregulation of CECR1 expression has been linked to Cat Eye Syndrome and other genetic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human CECR1 ELISA Kit provides researchers with a powerful tool for investigating the role of CECR1 in health and disease, offering valuable insights into its potential as a target for novel treatment strategies.
Human CECR1 (Cat Eye Syndrome Chromosome Region, Candidate 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CECR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CECR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CECR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CECR1. You can calculate the concentration of Human CECR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
93-105
98-113
Average (%)
93
99
104
1:4
Range (%)
89-105
86-96
85-98
Average (%)
96
91
91
1:8
Range (%)
87-101
82-93
82-96
Average (%)
92
86
88
1:16
Range (%)
90-104
80-92
87-100
Average (%)
96
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.4
8.5
1.1
2.52
8.65
Standard deviation
0.06
0.14
0.29
0.07
0.11
0.29
C V (%)
5.45
5.83
3.41
6.36
4.37
3.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CECR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CECR1 in samples. No significant cross-reactivity or interference between Human CECR1 and analogues was observed.
