Human CDK2 Recombinant Protein (RPPB2460)

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SKU:
RPPB2460
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
CDK2
Synonyms:
Cyclin-dependent kinase 2
Synonyms:
Cell division protein kinase 2
Synonyms:
p33 protein kinase
Synonyms:
CDK2
Source:
Escherichia Coli
Uniprot:
P24941
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Description

Product Name:Human CDK2 Recombinant Protein
Product Code:RPPB2460
Size:10µg
Species:Human
Target:CDK2
Synonyms:Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, p33(CDK2).
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:CDK2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLLE HHHHHH

Cyclin-dependent kinase 2 (CDK2) belongs the Ser/Thr protein kinase family. CDK2 is highly parallel to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is limited to the G1-S phase, and is vital for cell cycle G1/S phase transition. The CDK2 protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 activity is also regulated by protein phosphorylation.

CDK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids (1-298 a.a.) and having a molecular mass of 35kDa.CDK2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.22; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Cajal body; centrosome; chromosome, telomeric region; X chromosome; condensed chromosome; cytosol; Y chromosome; nucleoplasm; transcription factor complex; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; endosome

Molecular Function:cyclin binding; protein binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; meiosis; mitosis; positive regulation of transcription, DNA-dependent; histone phosphorylation; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; Ras protein signal transduction; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle; blood coagulation; centrosome duplication; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation

NCBI Summary:This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Code:P24941
NCBI GenInfo Identifier:116051
NCBI Gene ID:1017
NCBI Accession:P24941.2
UniProt Secondary Accession:P24941,O75100, A8K7C6,
UniProt Related Accession:P24941
Molecular Weight:30,035 Da
NCBI Full Name:Cyclin-dependent kinase 2
NCBI Synonym Full Names:cyclin-dependent kinase 2
NCBI Official Symbol:CDK2  
NCBI Official Synonym Symbols:CDKN2; p33(CDK2)  
NCBI Protein Information:cyclin-dependent kinase 2; p33 protein kinase; cdc2-related protein kinase; cell division protein kinase 2
UniProt Protein Name:Cyclin-dependent kinase 2
UniProt Synonym Protein Names:Cell division protein kinase 2; p33 protein kinase
Protein Family:Cyclin-dependent kinase
UniProt Gene Name:CDK2  
UniProt Entry Name:CDK2_HUMAN
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