Human CDA Recombinant Protein (RPPB1479)
- SKU:
- RPPB1479
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- CDA
- Synonyms:
- Cytidine deaminase
- Cytidine aminohydrolase
- CDA
- CDD
- Source:
- Escherichia Coli
- Uniprot:
- P32320
Description
| Product Name: | Human CDA Recombinant Protein |
| Product Code: | RPPB1479 |
| Size: | 20µg |
| Species: | Human |
| Target: | CDA |
| Synonyms: | Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CDA solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0), 1mM DTT, 2mM EDTA, 100mM NaCl and 40% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ |
| Biological Activity: | Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C. |
Cytidine deaminase (CDA) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. CDA is one of several deaminases responsible for maintaining the cellular pyrimidine pool. CDA also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. CDA can form homotetramers and is generally expressed in granulocytes. Mutations in the CDA gene are linked to decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.
CDA Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 a.a.) and having a molecular mass of 18.3kDa. The CDA is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.5.4.5 Chromosomal Location of Human Ortholog: 1p36.2-p35 Cellular Component: extracellular region; cytosol Molecular Function:protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization |
| NCBI Summary: | This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P32320 |
| NCBI GenInfo Identifier: | 1705718 |
| NCBI Gene ID: | 978 |
| NCBI Accession: | P32320.2 |
| UniProt Related Accession: | P32320 |
| Molecular Weight: | |
| NCBI Full Name: | Cytidine deaminase |
| NCBI Synonym Full Names: | cytidine deaminase |
| NCBI Official Symbol: | CDA |
| NCBI Official Synonym Symbols: | CDD |
| NCBI Protein Information: | cytidine deaminase |
| UniProt Protein Name: | Cytidine deaminase |
| UniProt Synonym Protein Names: | Cytidine aminohydrolase |
| Protein Family: | Colicin |
| UniProt Gene Name: | CDA |
| UniProt Entry Name: | CDD_HUMAN |
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