CD8B (CD8b molecule); also known as P37 and LEU2; contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen; acting as a coreceptor; and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains; or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. P37 gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene; also located on chromosome 2; has been identified. CD8 is thought to play a role in the process of T-cell mediated killing.
Product Name:
Human CD8 beta/CD8B Recombinant Protein (RPES4198)
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD8B isoform 1 (P10966-1) extracellular domain (Met 1-Pro 170) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 93 % as determined by reducing SDS-PAGE.
Mol Mass:
44 kDa
AP Mol Mass:
50 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
