Human CD42 (Cluster of Differentiation 42) ELISA Kit
The Human CD42 (Cluster of Differentiation 42) ELISA Kit is specifically designed for the accurate quantification of CD42 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for various research applications.CD42, also known as glycoprotein Ib alpha, is a critical receptor protein involved in platelet adhesion and thrombus formation. Dysregulation of CD42 expression has been implicated in various hematologic disorders such as von Willebrand disease and thrombocytopenia.
Therefore, the Human CD42 ELISA Kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.Overall, this ELISA kit offers researchers a reliable method for quantifying CD42 levels in human samples, facilitating further understanding of its role in platelet function and hematologic disorders.
Product Name:
Human CD42 (Cluster of Differentiation 42) ELISA Kit
SKU:
HUES01428
Target:
Human CD42 (Cluster of Differentiation 42)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD42. You can calculate the concentration of Human CD42 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
93-109
87-99
Average (%)
99
101
92
1:4
Range (%)
93-104
81-93
82-95
Average (%)
98
87
89
1:8
Range (%)
93-105
81-93
84-95
Average (%)
100
85
90
1:16
Range (%)
85-97
82-96
87-102
Average (%)
92
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.74
29.12
89.27
10.25
27.82
96.22
Standard deviation
0.59
1.64
3.6
0.57
1.41
3.29
C V (%)
5.49
5.63
4.03
5.56
5.07
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CD42 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD42 in samples. No significant cross-reactivity or interference between Human CD42 and analogues was observed.
