Human CD3D Recombinant Protein (RPPB3017)
- SKU:
- RPPB3017
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- CD3D
- Synonyms:
- CD3d Antigen
- Delta Polypeptide (TiT3 Complex)
- Source:
- Sf9 Insect cells
- Uniprot:
- P04234
Description
| Product Name: | Human CD3D Recombinant Protein |
| Product Code: | RPPB3017 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD3D |
| Synonyms: | CD3d Molecule, CD3d Antigen, Delta Polypeptide (TiT3 Complex), CD3d Molecule, Delta (CD3-TCR Complex), T-Cell Receptor T3 Delta Chain, T3D, T-Cell Surface Glycoprotein CD3 Delta Chain, CD3 Antigen, Delta Subunit, OKT3, Delta Chain, CD3d Antigen, CD3 Delta, CD3-DELTA, IMD19, CD3D. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CD3D protein solution (0.5mg/ml) contains 10% glycerol & Phosphate buffered saline (pH7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | FKIPIEELED RVFVNCNTSI TWVEGTVGTL LSDITRLDLG KRILDPRGIY RCNGTDIYKD KESTVQVHYR MCQSCVELDP ATVALEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH |
CD3D, also known as Delta Polypeptide (TiT3 Complex), is a single-pass type 1 membrane protein. CD3D jointly with CD3-zeta, CD3-gamma, CD3-epsilon and the T-cell receptor alpha/beta and gamma/delta heterodimers, creates the T-cell receptor-CD3 complex. Once antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transferred through the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. Likewise, CD3D takes an important part in adaptive immune response and participates in thymocyte differentiation.
CD3D produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (22-105 a.a.) and fused to a 239 aa hIgG-His-tag at C-terminus containing a total of 323 amino acids and having a molecular mass of 36.5kDa.CD3D shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q23 Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex Molecular Function:transmembrane receptor activity; protein heterodimerization activity; transcription coactivator activity Biological Process: regulation of immune response; cell surface receptor linked signal transduction; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; T cell differentiation; positive thymic T cell selection Disease: Immunodeficiency 19 |
| NCBI Summary: | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P04234 |
| NCBI GenInfo Identifier: | 115985 |
| NCBI Gene ID: | 915 |
| NCBI Accession: | P04234.1 |
| UniProt Secondary Accession: | P04234,A8MVP6, |
| UniProt Related Accession: | P04234 |
| Molecular Weight: | 171 |
| NCBI Full Name: | T-cell surface glycoprotein CD3 delta chain |
| NCBI Synonym Full Names: | CD3d molecule, delta (CD3-TCR complex) |
| NCBI Official Symbol: | CD3D |
| NCBI Official Synonym Symbols: | T3D; IMD19; CD3-DELTA |
| NCBI Protein Information: | T-cell surface glycoprotein CD3 delta chain; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex) |
| UniProt Protein Name: | T-cell surface glycoprotein CD3 delta chain |
| UniProt Synonym Protein Names: | T-cell receptor T3 delta chain; CD_antigen: CD3d |
| Protein Family: | CD302 antigen |
| UniProt Gene Name: | CD3D |
| UniProt Entry Name: | CD3D_HUMAN |
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