Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31),is a heavily glycosylated transmembrane protein belonging to theimmunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-likedomains, a transmembrane domain, and a 118 aacytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes,macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through itsextracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosinephosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold ofT cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a keyinhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.
Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
65.3 kDa
AP Mol Mass:
85-95 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
