Human CD30L (Cluster of Differentiation 30 Lligand) CLIA Kit
The Human CD30L (Cluster of Differentiation 30 Ligand) CLIA Kit is specifically designed for the sensitive and accurate measurement of CD30 ligand levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for various research applications.CD30 ligand is a protein that plays a crucial role in immune responses, particularly in regulating the function of T cells. Dysregulation of CD30 ligand has been associated with various inflammatory diseases, autoimmune disorders, and certain types of cancer.
Therefore, measuring CD30 ligand levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies.Overall, the Human CD30L CLIA Kit provides researchers with a powerful tool to investigate the role of CD30 ligand in health and disease, ultimately advancing our understanding of immune function and potential therapeutic interventions.
Product Name:
Human CD30L (Cluster of Differentiation 30 Lligand) CLIA Kit
SKU:
HUES00922
Target:
Human CD30L (Cluster of Differentiation 30 Lligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD30L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD30L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD30L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD30L. You can calculate the concentration of Human CD30L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
84-98
86-99
Average (%)
90
91
93
1:4
Range (%)
90-101
101-116
101-113
Average (%)
95
107
107
1:8
Range (%)
102-119
98-115
102-115
Average (%)
109
106
108
1:16
Range (%)
87-100
90-105
87-101
Average (%)
93
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.98
58.34
147.25
20.43
63.56
146.66
Standard deviation
1.91
6.85
12.84
2.33
5.94
16.62
C V (%)
10.06
11.74
8.72
11.4
9.35
11.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD30L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD30L in samples. No significant cross-reactivity or interference between Human CD30L and analogues was observed.
