The Human CD28 Monoclonal Antibody FITC Conjugated (CD28-2) (AGel2020) is a vital tool for researchers studying CD28, a co-stimulatory molecule essential for T cell activation and immune response regulation. This antibody, engineered for high specificity and sensitivity, is ideal for flow cytometry applications, allowing for precise detection and analysis of CD28 expression on immune cells.CD28 plays a crucial role in mediating T cell activation and promoting immune responses, making it a key target for understanding and manipulating immune function in various diseases, including cancer, autoimmune disorders, and infectious diseases. The FITC conjugation of this monoclonal antibody enhances its performance for flow cytometry, enabling researchers to investigate CD28 expression levels on different T cell subsets and evaluate their functional implications.
With its reliable performance and validated application in flow cytometry, the Human CD28 Monoclonal Antibody FITC Conjugated (CD28-2) (AGel2020) is a valuable tool for advancing research in immunology, cancer biology, and autoimmune diseases. By facilitating the precise detection and analysis of CD28 expression, this antibody contributes to a deeper understanding of T cell activation mechanisms and immune response regulation, ultimately paving the way for the development of novel therapeutic strategies targeting CD28-related pathways.
Product Title:
FITC Anti-Human CD28 Antibody [CD28.2]
SKU:
AGEL2020
Size:
20 Tests, 100 Tests, 200 Tests
Clone No:
CD28.2
Synonyms:
PAS2;MNS;HGpMiX;GYPA;GPSAT;
Host Species:
Mouse
Reactivity:
Human
Application:
FCM
Isotype:
Mouse IgG1, κ
Isotype Control:
FITC Mouse IgG1, κ Isotype Control[MOPC-21]
Form:
Liquid
Conjugation:
FITC
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P10747
Gene ID:
940
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
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