The Human CD235 Monoclonal Antibody (PE Conjugated) (HIR2) is a specialized research tool that targets CD235, a cell surface antigen known for its role in blood group typing and red blood cell differentiation. This monoclonal antibody is specifically designed to detect and analyze CD235 in human samples, making it an invaluable tool for studies in hematology, transfusion medicine, and blood-related disorders.CD235, also known as glycophorin A, is a key marker on red blood cells that plays a crucial role in determining blood type and compatibility. By targeting CD235 with this monoclonal antibody conjugated to PE, researchers can accurately identify and characterize red blood cells in various experimental settings.
This antibody is validated for use in flow cytometry and immunofluorescence applications, providing reliable and reproducible results for studying red blood cell biology and related clinical conditions.Researchers investigating conditions such as anemia, hemolytic disorders, and blood transfusion compatibility will find the Human CD235 Monoclonal Antibody (PE Conjugated) (HIR2) to be an essential tool in their research arsenal. Its high specificity and sensitivity make it a valuable resource for advancing our understanding of red blood cell biology and improving clinical diagnostics and treatments related to blood disorders.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P02724 P06028
Gene ID:
2993
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.