The Human CD235 Monoclonal Antibody (APC Conjugated) (HIR2-AGEL1094) is a cutting-edge tool for researchers studying CD235, a crucial cell surface marker involved in various biological processes. This monoclonal antibody, specifically designed for human samples, offers high reactivity and specificity, making it a reliable choice for flow cytometry and immunofluorescence applications.CD235, also known as glycophorin A, is a key player in red blood cell development and function. Its presence on the surface of erythroid cells makes it a valuable marker for studying hematopoiesis, blood disorders, and transfusion medicine.
The APC conjugation of this antibody enables easy and efficient detection of CD235, allowing researchers to analyze its expression in different cell types and experimental conditions.Understanding the role of CD235 is essential for unraveling the complexities of erythropoiesis and related disorders such as anemia and hemolytic diseases. This monoclonal antibody opens new avenues for investigating the function and regulation of CD235, providing insights that can contribute to advancements in both basic science and clinical research in the field of hematology.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P02724 P06028
Gene ID:
2993
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.