The Human CD235 Monoclonal Antibody (AF647 Conjugated) HIR2 (AGEL1098) offered by AssayGenie is a cutting-edge tool for researchers studying CD235, a cell surface antigen involved in red blood cell biology. This antibody is specifically designed for use in flow cytometry and immunofluorescence applications, allowing for precise and sensitive detection of CD235 in human samples.CD235, also known as glycophorin A, plays a crucial role in erythropoiesis and red blood cell development. By targeting this protein, researchers can gain insights into blood disorders, erythrocyte function, and related hematological conditions. The AF647 conjugation of this monoclonal antibody provides enhanced signal detection in fluorescence-based experiments, ensuring reliable and reproducible results in diverse research settings.
Utilizing the Human CD235 Monoclonal Antibody (AF647 Conjugated) HIR2 (AGEL1098) from AssayGenie enables detailed analysis of CD235 expression and localization in a variety of biological samples. Its high specificity and sensitivity make it an indispensable tool for investigations into erythropoiesis, hematology, and related areas of research, facilitating a deeper understanding of red blood cell biology and associated pathologies.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P02724 P06028
Gene ID:
2993
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.