The Human CD235 Monoclonal Antibody (AF488 Conjugated) (HIR2) is a valuable tool for researchers studying CD235, a cell surface molecule involved in various biological processes. This antibody, conjugated with AF488 for fluorescent detection, is specifically designed for use in flow cytometry and fluorescence microscopy applications. CD235, also known as Glycophorin A, is a key protein on red blood cells that plays a crucial role in blood group antigens and erythrocyte development. The CD235 Monoclonal Antibody allows for precise detection and visualization of CD235 expression in human samples, providing insights into hematological disorders, blood transfusion compatibility, and erythropoiesis research.
By targeting CD235, researchers can better understand the mechanisms underlying blood cell development and function, leading to advancements in diagnostics and therapies for conditions such as anemia, hemolytic diseases, and other blood-related disorders. The CD235 Monoclonal Antibody (AF488 Conjugated) offers a reliable and sensitive tool for investigating CD235 biology in a range of experimental settings.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P02724 P06028
Gene ID:
2993
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.