Carbohydrate sulfotransferase 15; also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST; B-cell RAG-associated gene protein; CHST15 and BRAG; is a single-pass type I I membrane protein which belongs to the sulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen; lymph node; tonsil; bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B; pre-B; and mature B-cell lines; it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4;6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence; GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4); to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression.
Product Name:
Human CD16b/FCGR3B Recombinant Protein (RPES4127)
Product Code:
RPES4127
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, FCG3, FCGR3, CD16b and FCGR4B,FCRIII,FCRIIIb
Accession:
O75015-1
Sequence:
Met 18-Gly 193
Fusion tag:
Activity:
1.Using the Octet RED System; the affinity constant (Kd) of CD16b bound to Human IgG1 was 80nM.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human CD16b (O75015-1) (Met 18-Gly 193) was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20 kDa
AP Mol Mass:
22 kDa
Formulation:
Lyophilized from sterile 20mM Tris; 50mM NaCl; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
