Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.
Product Name:
Human CD122/IL-2RB Recombinant Protein (RPES1420)
Product Code:
RPES1420
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD122,IL15RB,P70-75
Accession:
NP_000869.1
Sequence:
Met 1-Asp 239
Fusion tag:
C-Fc
Activity:
1.Measured by its ability to bind biotinylated recombinant rat IL2 in a functional ELISA.2. Using the Octet RED System, the affinity constant (Kd) of Human IL2RB-Fc bound to Human IL2 was 0.8nM.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Asp 239) of human IL2 R? (NP_000869.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
51.6 kDa
AP Mol Mass:
60-65 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
