Human CD109 (Cluster of Differentiation 109) ELISA Kit
The Human CD109 (Cluster of Differentiation 109) ELISA Kit is a powerful tool for the precise measurement of CD109 levels in human serum, plasma, and cell culture supernatants. Featuring advanced sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for various research purposes.CD109 is a vital cell surface glycoprotein that plays a key role in regulating cell growth, differentiation, and immune response.
Its involvement in various diseases, such as cancer and inflammatory disorders, makes it a valuable biomarker for understanding disease mechanisms and exploring potential therapeutic approaches.With the Human CD109 ELISA Kit, researchers can gain deeper insights into the role of CD109 in health and disease, paving the way for new discoveries and advancements in medical research.
Product Name:
Human CD109 (Cluster of Differentiation 109) ELISA Kit
SKU:
HUES01892
Target:
Human CD109 (Cluster of Differentiation 109)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD109. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD109 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD109, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD109. You can calculate the concentration of Human CD109 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
88-104
98-114
Average (%)
103
95
106
1:4
Range (%)
86-99
84-96
89-99
Average (%)
93
89
94
1:8
Range (%)
92-107
81-92
87-100
Average (%)
99
87
92
1:16
Range (%)
91-103
84-96
86-100
Average (%)
98
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
3.13
8.16
1.02
2.93
8.36
Standard deviation
0.06
0.16
0.43
0.06
0.13
0.44
C V (%)
5.5
5.11
5.27
5.88
4.44
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CD109 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD109 in samples. No significant cross-reactivity or interference between Human CD109 and analogues was observed.
