Human CBX3 Recombinant Protein (RPPB2989)

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SKU:
RPPB2989
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
CBX3
Synonyms:
Chromobox homolog 3 (Drosophila HP1 gamma)
Synonyms:
Heterochromatin protein 1 homolog gamma
Synonyms:
Modifier 2 protein
Synonyms:
HP1 gamma homolog
Source:
Escherichia Coli
Uniprot:
Q13185
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Description

Product Name:Human CBX3 Recombinant Protein
Product Code:RPPB2989
Size:20µg
Species:Human
Target:CBX3
Synonyms:Chromobox homolog 3 (Drosophila HP1 gamma), Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma homolog, HECH, HP1Hs-gamma.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CBX3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ

CBX3 takes part in transcriptional silencing in heterochromatin-like complexes. CBX3 identifies and binds histone H3 tails methylated at 'Lys-9', thereby create an epigenetic repression. Additionally, CBX3 takes part in the formation of functional kinetochore by interacting with MIS12 complex proteins.

CBX3 Human Recombinant produced in E. coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.4kDa.CBX3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: chromatin; chromocenter; chromosome, pericentric region; nuclear chromosome, telomeric region; nuclear envelope; nuclear heterochromatin; nuclear inner membrane; nucleus; spindle

Molecular Function:enzyme binding; identical protein binding; protein binding; protein domain specific binding

Biological Process: chromatin remodeling; negative regulation of transcription, DNA-dependent; rhythmic process; transcription, DNA-dependent

NCBI Summary:At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Code:Q13185
NCBI GenInfo Identifier:116241284
NCBI Gene ID:11335
NCBI Accession:Q13185.4
UniProt Secondary Accession:Q13185,Q96CD7, Q99409, Q9BVS3, Q9P0Z6,
UniProt Related Accession:Q13185
Molecular Weight:20,811 Da
NCBI Full Name:Chromobox protein homolog 3
NCBI Synonym Full Names:chromobox 3
NCBI Official Symbol:CBX3  
NCBI Official Synonym Symbols:HECH; HP1-GAMMA; HP1Hs-gamma  
NCBI Protein Information:chromobox protein homolog 3
UniProt Protein Name:Chromobox protein homolog 3
UniProt Synonym Protein Names:HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein
Protein Family:Chromobox protein
UniProt Gene Name:CBX3  
UniProt Entry Name:CBX3_HUMAN
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