The Human CASP7 (Caspase-7) CLIA Kit is specifically designed for the accurate measurement of caspase-7 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Caspase-7 is a key protein involved in programmed cell death, playing a crucial role in apoptosis regulation. Dysregulation of caspase-7 activity has been implicated in various diseases, including cancer, Alzheimer's disease, and autoimmune disorders, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.
With its reliable performance and ease of use, the Human CASP7 CLIA Kit is a valuable tool for researchers studying cell death pathways and exploring potential treatment strategies for caspase-7 related diseases.
Product Name:
Human CASP7 (Caspase 7) CLIA Kit
SKU:
HUES00437
Target:
Human CASP7 (Caspase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP7. You can calculate the concentration of Human CASP7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
99-113
92-106
Average (%)
101
106
97
1:4
Range (%)
94-106
85-97
101-114
Average (%)
99
92
108
1:8
Range (%)
96-111
97-112
101-116
Average (%)
102
103
109
1:16
Range (%)
100-115
102-115
98-113
Average (%)
106
109
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.96
598.3
1584.92
187.61
614.81
1709.91
Standard deviation
16.87
61.74
154.21
22.14
58.22
124.14
C V (%)
8.48
10.32
9.73
11.8
9.47
7.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP7 in samples. No significant cross-reactivity or interference between Human CASP7 and analogues was observed.
