The Human Casp1 (Caspase-1) ELISA Kit is specifically designed for the accurate measurement of Caspase-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Caspase-1 is an important enzyme involved in the inflammatory response and immune system regulation. It plays a key role in the activation of the pro-inflammatory cytokines and processes such as pyroptosis.
Dysregulation of Caspase-1 has been linked to various diseases, including autoimmune disorders, infectious diseases, and neurodegenerative conditions.This ELISA kit provides researchers with a valuable tool for studying the role of Caspase-1 in these diseases, as well as potential therapeutic interventions. The user-friendly format and accurate results make it a convenient and reliable choice for laboratories conducting immunoassays.
Product Name:
Human CASP1 (Caspase 1) ELISA Kit
SKU:
HUES01303
Target:
Human CASP1 (Caspase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP1. You can calculate the concentration of Human CASP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
88-104
94-106
Average (%)
90
95
99
1:4
Range (%)
91-103
81-95
83-95
Average (%)
96
87
88
1:8
Range (%)
92-104
81-93
88-100
Average (%)
97
87
94
1:16
Range (%)
85-99
81-94
83-96
Average (%)
92
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
249.4
496.4
2112.03
263.99
457.28
2267.35
Standard deviation
14.69
26.86
111.94
17.66
20.3
83.89
C V (%)
5.89
5.41
5.3
6.69
4.44
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CASP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed.
