Peripheral plasma membrane protein CASK; also known as calcium/calmodulin-dependent serine protein kinase; CASK and LIN2; is a nucleus; cytoplasm and cell membrane protein which belongs to the MAGUK family. CASK / LIN2 contains one guanylate kinase-like domain; two L27 domains; one PDZ (DHR) domain; one protein kinase domain and one SH3 domain. CASK / LIN2 is ubiquitously expressed. Expression of CASK / LIN2 is significantly greater in brain relative to kidney; lung; and liver and in fetal brain and kidney relative to lung and liver. CASK / LIN2 is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. CASK / LIN2 contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. It binds to cell-surface proteins; including amyloid precursor protein; neurexins and syndecans. CASK / LIN2 may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Defects in CASK are also the cause of FG syndrome type 4 which is an X-linked disorder characterized by mental retardation; relative macrocephaly; hypotonia and constipation.
Product Name:
Human CASK Kinase Recombinant Protein (RPES4774)
Product Code:
RPES4774
Size:
20µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
CAGH39,CAMGUK,CMG,FGS4,LIN2,MICPCH,MRXSNA,TNRC8
Accession:
O14936-4
Sequence:
Ala 2-Tyr 898
Fusion tag:
N-His & GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CASK isoform 4 (O14936-4) (Ala 2-Tyr 898) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
